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Peritoneal dialysate fluid composition determines heat shock protein expression patterns in human mesothelial cells
K Arbeiter1, B Bidmon, M Endemann
1Kinderdialyse, Department of Pediatrics, Universitätsklinik für Kinder und Jugendheilkunde, AKH Wien, Währinger Gürtel 18-20, A-1090 Vienna, Austria.
Kidney International
|November 13, 2001
Summary
Heat shock protein 72 (HSP-72) is a key indicator of mesothelial cell injury from peritoneal dialysis fluids (PDF). Its expression is increased by acidic pH and glucose degradation products in PDF, suggesting HSP-72 can assess PDF biocompatibility.
Area of Science:
- Cellular Biology
- Biomaterials Science
- Nephrology
Background:
- Peritoneal dialysis fluids (PDF) can cause mesothelial cell injury due to poor biocompatibility.
- Heat shock proteins (HSPs) are involved in cellular stress responses.
Purpose of the Study:
- To investigate differential induction of heat shock proteins (HSP-27, HSP-72, HSP-90) in mesothelial cells exposed to PDF.
- To determine if modulating PDF physicochemical properties affects HSP induction.
Main Methods:
- Human mesothelial cells (Met5A, primary) were exposed to standard PDF (CAPD2, CAPD3) or a modified PDF (BALANCE).
- Western blotting and immunocytochemistry assessed HSP-27, HSP-72, and HSP-90 expression and HSP-72 localization.
- Specific PDF components (acidic pH, glucose degradation products) were evaluated for their role in HSP induction.
Main Results:
- Mesothelial cells constitutively express HSP-27, HSP-72, and HSP-90.
- Acidic PDF (CAPD2, CAPD3) significantly upregulated HSP-72 expression.
- Modified PDF (BALANCE) did not alter HSP-27 or HSP-72 levels.
- Acidic pH and glucose degradation products were identified as key mediators of HSP-72 upregulation.
Conclusions:
- HSP expression analysis offers a novel method for assessing PDF biocompatibility.
- HSP-72 is a sensitive and accurate biomarker for early-stage mesothelial cell injury induced by PDF.