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Updated: Aug 19, 2026
Scalable High Throughput Selection From Phage-displayed Synthetic Antibody Libraries
Published on: January 17, 2015
Isolation of human scFv antibody fragments against ABO blood group antigens from a phage display library
E Santos-Esteban1, E Curiel-Quesada
1Departamento de Bioquímica, Escuela Nacional de Ciencias Biológicas del I. P. N., Prol. de Carpio y Plan de Ayala, CP 11340, México D.F., México.
Background And Objectives:
Phage display has proven very useful for the isolation of antibodies against a number of antigens. We used this technology to isolate scFv antibody fragments against A and B red blood cell antigens.
Materials And Methods:
Phages from a phage display library were selected using unmodified red blood cells as a source of antigen. Bound phages were absorbed onto cells lacking the antigen of interest and used to infect Escherichia coli cells. Phages were rescued and assayed for specificity by enzyme-linked immunosorbent assay (ELISA).
Results:
After several rounds of panning and subtraction on red blood cells, one anti-A and one anti-B human scFv antibody fragments were isolated.
Conclusion:
Isolation of anti-A and anti-B scFv antibody fragments on whole cells is an alternative method of obtaining antibodies against native cell-surface antigens.
