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Comparison of two blastocyst culture systems: coculture on Vero cells and sequential media
S G d'Estaing1, J Lornage, S Hadj
1Département de Médecine de la Reproduction, Centre hospitalier de l'Université de Biologie de la reproduction, Hôpital Edouard Herriot, Lyon, France. sge@rockefeller.univ-lyon1.fr
Objective:
To compare two blastocyst culture systems: culture on Vero cells and sequential media in the same time period.
Design:
Retrospective study.
Setting:
Institutional practice assisted reproductive technology center.
Patient(S):
Ninety-nine selected patients undergoing IVF with blastocyst culture.
Intervention(S):
In vitro fertilization and transfer of at least one expanded blastocyst after culture using either Vero cells or sequential media (S(2) medium).
Main Outcome Measure(S):
Blastocyst, pregnancy, and implantation rates.
Result(S):
The blastocyst formation rate (41.6% vs. 31.6%) is significantly increased with sequential media compared with the coculture system, but there is no statistically significant difference in the pregnancy and implantation rates (39.5% vs. 38.6% and 23.7% vs. 15.1%, respectively).
Conclusion(S):
Blastocyst culture on sequential media offers a better dynamic of embryo development (22% vs. 13.6% blastocyst at day 5). There was no statistically significant difference between the two groups in the take-home baby rate (18 vs. 12).