Related Experiment Video

Updated: Aug 8, 2026

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
07:23

Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

Published on: June 15, 2016

Regulation of STAT protein synthesis by c-Cbl

W A Blesofsky1, K Mowen, R M Arduini

  • 1Department of Biology and UCSD Cancer Center, University of California San Diego, La Jolla, California 92093-0322, USA.

Oncogene
|November 13, 2001
PubMed

Insights

The Cbl protein family regulates Signal Transducers and Activators of Transcription (STAT) protein expression. c-Cbl deficiency increases STAT1 and STAT5 protein levels by enhancing STAT1 protein synthesis, not degradation.

Area of Science:

  • Molecular Biology
  • Cellular Signaling
  • Immunology

Background:

  • Cytokines and growth factors activate Signal Transducers and Activators of Transcription (STAT) proteins to induce gene expression.
  • While STAT protein activation is well-studied, the regulation of STAT protein expression levels remains less understood.

Purpose of the Study:

  • To investigate the role of c-Cbl in regulating STAT protein expression.
  • To elucidate the mechanism by which c-Cbl influences STAT protein levels.

Main Methods:

  • Analysis of STAT protein and mRNA levels in c-Cbl-deficient murine embryonic fibroblasts.
  • Assessment of STAT1 protein half-life and synthesis rates.
  • Comparison of STAT1, STAT2, STAT3, Jak1, and Tyk2 expression in wild-type and c-Cbl-deficient cells.

Main Results:

  • c-Cbl-deficient cells showed significantly increased levels of STAT1 and STAT5 proteins.
  • STAT1 and STAT5 expression were regulated by c-Cbl, while STAT2, STAT3, Jak1, and Tyk2 were not.
  • c-Cbl deficiency increased STAT1 protein synthesis rate without affecting its half-life or mRNA levels.

Conclusions:

  • c-Cbl plays a novel role in regulating STAT protein expression, specifically impacting STAT1 and STAT5 levels.
  • The Cbl protein family provides an additional layer of control over STAT1 function through regulation of protein synthesis.
  • This study reveals a new biological function for the Cbl protein family in cellular signaling pathways.

More Related Videos

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts11:19

Merging Absolute and Relative Quantitative PCR Data to Quantify STAT3 Splice Variant Transcripts

Published on: October 9, 2016

SorLA and CLC:CLF-1-dependent Downregulation of CNTFRα as Demonstrated by Western Blotting, Inhibition of Lysosomal Enzymes, and Immunocytochemistry10:16

SorLA and CLC:CLF-1-dependent Downregulation of CNTFRα as Demonstrated by Western Blotting, Inhibition of Lysosomal Enzymes, and Immunocytochemistry

Published on: January 6, 2017

Related Concept Videos

Covalently Linked Protein Regulators02:04

Covalently Linked Protein Regulators

Proteins can undergo many types of post-translational modifications, often in response to changes in their environment. These modifications play an important role in the function and stability of these proteins. Covalently linked molecules include functional groups, such as methyl, acetyl, and phosphate groups, and also small proteins, such as ubiquitin. There are around 200 different types of covalent regulators that have been identified.
These groups modify specific amino acids in a protein.
Regulation of Expression Occurs at Multiple Steps02:24

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Regulation of Nuclear Protein Sorting01:45

Regulation of Nuclear Protein Sorting

Nuclear protein sorting regulates nucleus composition and gene expression, crucial for determining the fate of a eukaryotic cell. Hence, the entry and exit of molecules across the nuclear envelope is a tightly controlled process. Nuclear protein sorting can be inhibited by one of the following ways: 1) masking cargo signal sequences, 2) modifying the nuclear receptor's affinity for cargo, 3) controlling the nuclear pore size, 4) retaining the cargo during its transit to the cytosol or the...
The JAK-STAT Signaling Pathway01:20

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as  SH2...
Regulation of Expression at Multiple Steps01:23

Regulation of Expression at Multiple Steps

The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the addition of a...
Translational Regulation01:29

Translational Regulation

Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...