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Updated: Aug 4, 2026

Oral Biofilm Sampling for Microbiome Analysis in Healthy Children
Published on: December 31, 2017
[Contribution to the study of dental caries in 0-30-month-old infants]
S G de Barros1, A C Alves, L S Pugliese
1Faculdade de Odontologia, Universidade Federal da Bahia.
Insights
This study found that over half of young children in Brazilian day nurseries have early signs of tooth decay, with prevalence increasing significantly with age. Poor oral hygiene and biofilm are linked to higher rates of cavities in toddlers.
Area of Science:
- Pediatric Dentistry
- Cariology
- Public Health
Context:
- Evaluated oral health in 340 children aged 0-30 months in Salvador, Brazil.
- Assessed incipient carious lesions using clinical examination and parental questionnaires.
- Investigated caries risk factors including hygiene, diet, and fluoride utilization.
Purpose:
- To determine the prevalence and characteristics of early childhood caries (ECC) in a vulnerable population.
- To identify associations between oral health conditions and risk factors in toddlers.
- To inform targeted public health interventions for pediatric oral health.
Summary:
- A high prevalence of caries (55.3%) was observed, increasing with age (25% in 0-12 months, 71.03% in 25-30 months).
- Active white spots affected 49.7% of children, while 17.6% had cavitated lesions, predominantly on anterior teeth.
- Increased biofilm and sleep-time nursing were associated with higher caries rates, highlighting the need for early intervention.
Impact:
- Findings underscore the urgent need for early oral health interventions and preventive programs in childcare settings.
- Highlights the importance of parental education on oral hygiene and dietary habits to combat early childhood caries.
- Provides crucial data for public health policy development aimed at improving pediatric oral health outcomes in Brazil.
Abstract:
This study evaluated the oral health conditions of 340 children, aged 0-30 months (21.3 +/- 5.6)--54.4% of girls and 45.6% of boys--from 20 public day nurseries of Salvador (Brazil), as to the presence of incipient carious lesions. The exam was carried out by a single examiner, who utilized a mirror, a probe and a penlight. The teeth were wiped with gauzes in order to remove the dental plaque. A questionnaire was answered by the children's parents or caretakers in order to assess information regarding knowledge on caries, caries risk factors, socioeconomic status of the family and utilization of fluoride. Two hundred and twenty-nine answered questionnaires (67.35%) were obtained. The observed lesions were classified in five stages, according to their severity (C0-C4; active/inactive). The data were analyzed using the Epi-info 6.02. The prevalence of caries was 55.3% when all stages were registered: 25% for subjects aged 0-12 months, 51.18% for subjects aged 13-24 months and 71.03% for those aged 25-30 months (chi 2 = 25.31, p < 0.01). When only active white spots were considered, 49.7% of the children were affected; 17.6% of the children presented with cavitated lesions. Among the affected children, 90.96% had lesions only on anterior teeth: 80% of the lesions were white spots and 20%, cavities. No significant difference was observed between genders. The increased amount of biofilm was positively associated with dental decay in toddlers (chi 2 = 67.61, p < 0.01), and the number of affected children increased when the sleep-time nursing habit was present (chi 2 = 0.24, p = 0.62). The prevalence of lesions increased with age and with the number of erupted teeth (chi 2 = 25.31, p < 0.01; chi 2 = 122.95, p < 0.01). Early oral health attention, diagnosis of incipient lesions, as well as educative and preventive programs to change oral hygiene and dietary habits are suggested.
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