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Analysis of precocenes in the essential oil of Ageratum spp. by reverse-phase high-performance liquid chromatography
1Department of Chemistry and Biochemistry, Himachal Pradesh Krishi Vishvavidayalaya, Palampur, H.P., India. ivriplp@nde.vsnl.net.in
Abstract:
The chromenes precocene I (7-methoxy-2,2-dimethylchromene) and precocene II (6,7-methoxy-2,2-dimethylchromene) extracted from Ageratum houstonianum and A. conyzoides have been analysed by reverse-phase high-performance liquid chromatography. Separation was achieved using a C18 column eluted with a linear gradient of acetonitrile and water. Precocenes I and II in the essential oils were determined to be 270 +/- 23 and 482 +/- 34 mg/mL in A. houstonianum, and 687 +/- 21 and 20 +/- 2 mg/mL in A. conyzoides.
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