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Fish monocytes as a model for mycobacterial host-pathogen interactions.

S H El-Etr1, L Yan, J D Cirillo

  • 1Department of Veterinary and Biomedical Sciences, University of Nebraska at Lincoln, 68583-0905, USA.

Infection and Immunity
|November 14, 2001
PubMed
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A carp monocytic cell line (CLC) provides an effective in vitro model for studying Mycobacterium marinum virulence. Fish monocytes distinguish between pathogenic and nonpathogenic mycobacteria, aiding in identifying virulence factors.

Area of Science:

  • * Infectious Diseases
  • * Cell Biology
  • * Immunology

Background:

  • * Mycobacterium marinum is a fish and human pathogen closely related to Mycobacterium tuberculosis.
  • * It causes tuberculosis-like diseases in fish and amphibians.
  • * Understanding M. marinum virulence mechanisms is crucial for both human and animal health.

Purpose of the Study:

  • * To develop and utilize an in vitro model for studying M. marinum virulence factors.
  • * To investigate the interaction between M. marinum and fish monocytic cells.
  • * To explore the potential of fish monocytes in dissecting mycobacterial intracellular trafficking.

Main Methods:

  • * Development of an in vitro model using the carp monocytic cell line CLC (carp leukocyte culture).

Related Experiment Videos

  • * Comparative analysis of M. marinum and Mycobacterium smegmatis interaction with CLC cells.
  • * Assessment of mycobacterial entry, survival, replication, and lysosomal fusion inhibition in fish monocytes.

    • Main Results:

    • * Fish monocytes (CLC cells) differentiate between pathogenic (M. marinum) and nonpathogenic (M. smegmatis) mycobacteria.
    • * M. marinum exhibits significantly higher entry rates (40-60 fold) into fish monocytes compared to M. smegmatis.
    • * M. marinum survives and replicates within fish monocytes, while M. smegmatis is eliminated; M. marinum inhibits lysosomal fusion.

    Conclusions:

    • * The carp monocytic cell line (CLC) serves as a valuable in vitro model for M. marinum pathogenesis research.
    • * Fish monocytes effectively model host-pathogen interactions relevant to mycobacterial virulence.
    • * This model facilitates the identification and characterization of M. marinum virulence determinants and intracellular trafficking mechanisms.