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Related Experiment Videos

Fish monocytes as a model for mycobacterial host-pathogen interactions.

S H El-Etr1, L Yan, J D Cirillo

  • 1Department of Veterinary and Biomedical Sciences, University of Nebraska at Lincoln, 68583-0905, USA.

Infection and Immunity
|November 14, 2001
PubMed
Summary

A carp monocytic cell line (CLC) provides an effective in vitro model for studying Mycobacterium marinum virulence. Fish monocytes distinguish between pathogenic and nonpathogenic mycobacteria, aiding in identifying virulence factors.

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Area of Science:

  • * Infectious Diseases
  • * Cell Biology
  • * Immunology

Background:

  • * Mycobacterium marinum is a fish and human pathogen closely related to Mycobacterium tuberculosis.
  • * It causes tuberculosis-like diseases in fish and amphibians.
  • * Understanding M. marinum virulence mechanisms is crucial for both human and animal health.

Purpose of the Study:

  • * To develop and utilize an in vitro model for studying M. marinum virulence factors.
  • * To investigate the interaction between M. marinum and fish monocytic cells.
  • * To explore the potential of fish monocytes in dissecting mycobacterial intracellular trafficking.

Main Methods:

  • * Development of an in vitro model using the carp monocytic cell line CLC (carp leukocyte culture).

Related Experiment Videos

  • * Comparative analysis of M. marinum and Mycobacterium smegmatis interaction with CLC cells.
  • * Assessment of mycobacterial entry, survival, replication, and lysosomal fusion inhibition in fish monocytes.

    • Main Results:

    • * Fish monocytes (CLC cells) differentiate between pathogenic (M. marinum) and nonpathogenic (M. smegmatis) mycobacteria.
    • * M. marinum exhibits significantly higher entry rates (40-60 fold) into fish monocytes compared to M. smegmatis.
    • * M. marinum survives and replicates within fish monocytes, while M. smegmatis is eliminated; M. marinum inhibits lysosomal fusion.

    Conclusions:

    • * The carp monocytic cell line (CLC) serves as a valuable in vitro model for M. marinum pathogenesis research.
    • * Fish monocytes effectively model host-pathogen interactions relevant to mycobacterial virulence.
    • * This model facilitates the identification and characterization of M. marinum virulence determinants and intracellular trafficking mechanisms.