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High-sensitivity analysis of cyanide by capillary electrophoresis with fluorescence detection
S Chinaka1, S Tanaka, N Takayama
1Forensic Science Laboratory, Ishikawa Prefectural Police Headquarters, 2-1-1 Hirosaka, Kanazawa 920-8553, Japan.
Abstract:
A capillary electrophoretic method for a high-sensitivity analysis of cyanide has been developed. Cyanide was derivatized with 2,3-naphthalenedialdehyde and taurine to give a fluorescent product of 1-cyanobenz[f]isoindole. This compound was detected with high sensitivity by fluorescence detection. The detection limit was 0.1 ng/mL, and the calibration curve was linear over the range 0.1-200 ng/mL. The precision of the migration time of within-run assays (n = 6) of 1 ng/mL cyanide standard solution was 0.14%. The precision of the peak area for the same runs was 1.0%. This method was applicable to blood analysis. Detection of the cyanide derivative by UV was also examined.