Protection of N-methyl-N'-nitro-N-nitrosoguanidine-induced in vivo clastogenicity by aqueous garlic extract

The modulatory effects of garlic extract on the in vivo clastogenicity of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), a carcinogenic nitrosamine, were evaluated by quantification of micronuclei and chromosomal aberrations in metaphase cells from the bone marrow of male Wistar rats. A single intraperitoneal injection of MNNG (40 mg/kg bodyweight) was found to be clastogenic as revealed by the increased frequency of micronucleated polychromatic erythrocytes and chromosomal aberrations. Pretreatment with aqueous garlic extract (250 mg/kg bodyweight) for 5 days significantly reduced the frequencies of MNNG-induced micronuclei and chromosomal aberrations. The results demonstrate that administration of garlic extract protects against the clastogenic effects of MNNG.