Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Microarray analysis of gene expression in human donor corneas.

A S Jun¹, S H Liu, E H Koo

¹Cornea and External Disease Division, Wilmer Ophthalmological Institute, The Johns Hopkins Medical Institutions, Maumenee 317, 600 N Wolfe St, Baltimore, MD 21287, USA.

Archives of Ophthalmology (Chicago, Ill. : 1960)

|November 16, 2001

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

[Impact of cumulative atherogenic index of plasma exposure on the risk of new-onset cardiovascular disease in middle-aged and young populations].

Zhonghua xin xue guan bing za zhi·2026

Same author

[Correlations of foveal avascular zone area, acircularity index and vision before and after surgery for idiopathic macular epiretinal membrane].

[Zhonghua yan ke za zhi] Chinese journal of ophthalmology·2026

Same author

[Effects of allergens on the expression levels of interleukin 18, interleukin 18 binding protein a and interleukin 18 receptor α in the blood monocyte subtypes of patients with allergic asthma].

Zhonghua nei ke za zhi·2025

Same author

[Effects of acellular allogeneic dermis combined with autologous split-thickness skin grafts in repairing deep burn wounds in head, face, neck, and torso in children].

Zhonghua shao shang yu chuang mian xiu fu za zhi·2025

Same author

[Comparison of positive criteria for ATS/ERS bronchodiastolic tests in 2005 and 2021 with different degree of airway obstruction].

Zhonghua yi xue za zhi·2025

Same author

[A case of cirrhosis combined with myeloproliferative neoplasms].

Zhonghua gan zang bing za zhi = Zhonghua ganzangbing zazhi = Chinese journal of hepatology·2024

This study used microarray analysis to identify 1200 genes in human corneas, creating a database of corneal gene expression. This research advances understanding of corneal function and disease.

Area of Science:

Ophthalmology
Molecular Biology
Genomics

Background:

The cornea's gene expression profile is crucial for understanding its function and diseases.
Previous studies have not comprehensively cataloged corneal genes.

Purpose of the Study:

To identify genes expressed in human donor corneas using microarray analysis.
To establish a comprehensive database of human corneal gene expression for scientific accessibility.

Main Methods:

Complementary DNA (cDNA) library construction from human donor corneas.
Hybridization of biotin-labeled RNA to microarrays containing approximately 5600 human genes.
Confirmation of gene expression using reverse transcriptase polymerase chain reaction (RT-PCR).

Related Experiment Videos

Main Results:

Identified expression of 1200 genes in human corneas, with 85% assigned chromosomal loci.
Compiled a preliminary database of corneal gene expression, accessible via a website.
Discovered novel corneal localizations for six collagen genes and five apoptosis-related genes, including disease-associated genes.

Conclusions:

Microarray analysis provides a comprehensive preliminary database of human corneal gene expression.
Accessible data facilitates understanding of normal and abnormal corneal function.
Identification of specific genes offers insights into corneal diseases and potential therapeutic targets.