Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

beta-Casein adsorption at the silicon oxide--aqueous solution interface.

F Tiberg1, T Nylander, T J Su

  • 1Institute for Surface Chemistry, Box 5607, S-114 86 Stockholm, Sweden.

Biomacromolecules
|November 17, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Organizational deployment and on-site handling for forensic examination after exhumation.]

Fa yi xue za zhi·2026
Same author

Biosurfactant/surfactant mixing properties at the air-water interface: comparing rhamnolipids and sophorolipids mixed with the anionic surfactant sodium dodecyl benzene sulfonate.

Soft matter·2025
Same author

Promoting the adsorption of saponins at the hydrophilic solid-aqueous solution interface by the coadsorption with cationic surfactants.

Journal of colloid and interface science·2024
Same author

Manipulating the hydrophilic / hydrophobic balance in novel cationic surfactants by ethoxylation: The impact on adsorption and self-assembly.

Journal of colloid and interface science·2024
Same author

The adsorption and self-assembly of surfactant mixtures: How the detailed evaluation of adsorption properties provides access to the bulk behaviour.

Advances in colloid and interface science·2023
Same author

[A case of primary cardiac lymphoma diagnosed by intravenous right atrial catheter forceps biopsy].

Zhonghua xin xue guan bing za zhi·2022
Same journal

Stiffening and Toughening Protein Hydrogels by Tuning Electrostatic Interactions.

Biomacromolecules·2026
Same journal

<i>In Situ</i> Bulk and Interfacial Interlocking-Induced Highly Dynamically Entangled Hydrogel of Myocardium-Matching Mechanics, Electrophysiological Functions, and Robust Tissue Adhesion for Cardiac Repair.

Biomacromolecules·2026
Same journal

Eutectogel Electrodes with Self-powered Capability for Flexible Electrophysiological Sensor.

Biomacromolecules·2026
Same journal

Self-Reporting Supramolecular Coacervates Driven by Liquid-Liquid Phase Separation Enable Systemic Translocation and Photodynamic Bioprotection.

Biomacromolecules·2026
Same journal

Self-Assembed G-Quadruplex Nanowires for Energy Transfer over Micrometers.

Biomacromolecules·2026
Same journal

Modulating Macrophage Polarization via Core-Shell Nanofiber Membranes with Sequential Drug Delivery for Periodontal Regeneration.

Biomacromolecules·2026
See all related articles

Beta-casein protein adsorption onto silica surfaces is a slow process, forming a distinct asymmetric bilayer structure. This differs significantly from rapid adsorption on hydrophobic surfaces, indicating weaker binding and greater susceptibility to desorption and enzymatic degradation.

Area of Science:

  • Surface science
  • Protein adsorption
  • Biomaterials

Background:

  • Understanding protein adsorption is crucial for biomaterial design and food science.
  • Beta-casein is a key milk protein with significant implications in food and biomedical applications.
  • The nature of the substrate surface (hydrophilic vs. hydrophobic) influences protein adsorption behavior.

Purpose of the Study:

  • To investigate the time-dependent adsorption of beta-casein onto a silica (hydrophilic) surface.
  • To characterize the structural properties of the adsorbed beta-casein layer.
  • To compare the adsorption behavior on silica with that on hydrophobic substrates.

Main Methods:

  • Neutron reflectometry was employed to study the interfacial structure.
  • Reflectivity curves were analyzed using three-layer and two-layer models.

Related Experiment Videos

  • Desorption studies and enzymatic cleavage (endoproteinase Asp-N) were performed to assess binding strength and layer stability.

    • Main Results:

    • Beta-casein adsorption on silica is slow, forming an asymmetric bilayer with a dense inner layer and a hydrated outer layer.
    • The adsorbed layer on silica exhibits significant desorption upon rinsing and substantial reduction (over 80%) after enzymatic treatment.
    • Adsorption on silica contrasts with faster adsorption and greater stability observed on hydrophobic surfaces.

    Conclusions:

    • Beta-casein forms a distinct asymmetric bilayer on hydrophilic silica, unlike the monolayer on hydrophobic surfaces.
    • A fraction of adsorbed beta-casein on silica exhibits weak binding affinity, leading to pronounced desorption.
    • Neutron reflectometry is a viable technique for studying dynamic protein adsorption phenomena at interfaces.