Search research articles

Search research articles

Related Experiment Videos

Protein thioacylation. 1. Reagents design and synthesis.

G Levesque¹, P Arsène, V Fanneau-Bellenger

¹Centre de Recherche, Université de Bretagne-Sud, Rue de St Maudé, F-56325 Lorient, France.

Biomacromolecules

|November 17, 2001

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Proton Radiotherapy Offers Immune-Sparing Benefits in Glioblastoma Treatment.

International journal of radiation oncology, biology, physics·2026

Same author

Clear evidence for element partitioning effects in a Ti-6Al-4V alloy by the first-principles phase field method.

Journal of physics. Condensed matter : an Institute of Physics journal·2020

Same author

Progress towards rabies control and elimination in Vietnam.

Revue scientifique et technique (International Office of Epizootics)·2019

Same author

Prospective observational study comparing burn surgeons' estimations of wound healing after skin grafting to photo-assisted methods.

Burns : journal of the International Society for Burn Injuries·2019

Same author

Knowledge of Rabies Prevention in Vietnamese Public Health and Animal Health Workers.

Zoonoses and public health·2016

Same author

Awareness of rabies prevention and control measures among public health workers in Northern Vietnam.

Public health·2015

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Thioacylation offers a novel method for protein modification, rapidly forming thioamides with lysine residues at room temperature. This versatile chemical reaction enables the synthesis of diverse reagents for applications in enzyme modification and material science.

Area of Science:

Chemical Biology
Organic Chemistry
Materials Science

Background:

Protein chemical modification is crucial for biochemical research and biotechnology.
Existing methods often require specific catalysts or harsh reaction conditions.
There is a need for efficient and mild protein modification techniques.

Purpose of the Study:

To introduce thioacylation as a novel and efficient method for protein chemical modification.
To synthesize and characterize various thioacylating reagents.
To explore the applications of thioacylation in enzyme modification and new material development.

Main Methods:

Reaction of carboxylic dithioesters and -acids with aliphatic amines (lysine epsilon-amino groups).
Synthesis of monofunctional dithioesters with diverse chemical groups.

Related Experiment Videos

Synthesis of bifunctional dithioesters and dithioacids with varying spacer groups.

Main Results:

Selective and rapid thioamide formation at room temperature without catalysts.
Successful synthesis of a range of monofunctional and bifunctional thioacylating reagents.
Demonstrated potential for enzyme modification and creation of novel protein-based materials.

Conclusions:

Thioacylation is a versatile and efficient new strategy for protein modification.
The synthesized reagents offer diverse functionalities for various applications.
Bifunctional reagents show promise as cross-linking and coupling agents in protein chemistry.