Jove
Visualize
Contact Us

Related Experiment Videos

Bioflavonoid rescue of ascorbate at a membrane interface.

B Bandy1, E J Bechara

  • 1Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, SP, Brazil. bbandy@iq.usp.br

Journal of Bioenergetics and Biomembranes
|November 17, 2001
PubMed
Summary

Ascorbate protects cytochrome c from oxidation, but requires bioflavonoids to function within cell membranes. These compounds facilitate electron transfer, enhancing antioxidant defense.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Target deconvolution of a selective HepG2 cytotoxic hit identifies GSTP1 and TRAP1 as candidate molecular targets.

RSC advances·2026
Same author

Isozyme-specific inhibition of GSTP1-1: a crucial element in cancer-targeting drugs.

RSC medicinal chemistry·2025
Same author

Effects of halogens on European air-quality.

Faraday discussions·2017
Same author

2-[4-(4-Methoxyphenylcarbonyloxy)benzylidene]-6-dimethylaminomethyl cyclohexanone hydrochloride: a Mannich base which inhibits the growth of some drug-resistant strains of Mycobacterium tuberculosis.

Die Pharmazie·2010
Same author

The effect of some 1-[4-(2-diethylaminoethoxy)phenylcarbonyl]-3,5-bis (benzylidene)-4-piperidone methiodides and related compounds on respiration and swelling of rat liver mitochondria.

Die Pharmazie·2008
Same author

The role of oxidative stress in the development of congestive heart failure in a chicken genotype selected for rapid growth.

Avian pathology : journal of the W.V.P.A·2008
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Area of Science:

  • Biochemistry
  • Cell Biology
  • Oxidative Stress

Background:

  • Cytochrome c is a vital protein in cellular respiration and apoptosis.
  • Oxidative damage from reactive oxygen species (ROS) can impair cytochrome c function.
  • Ascorbate (Vitamin C) is a known antioxidant, but its efficacy in membrane environments is debated.

Purpose of the Study:

  • To investigate the protective effects of ascorbate on cytochrome c in the presence of lipid membranes.
  • To determine the role of bioflavonoids in enhancing ascorbate's antioxidant activity in a hydrophobic environment.
  • To elucidate the mechanism of bioflavonoid-mediated electron transfer from ascorbate.

Main Methods:

  • Experiments were conducted in aqueous solutions and liposomes mimicking mitochondrial membranes.

Related Experiment Videos

  • Protection of cytochrome c from bleaching by hydrogen peroxide and t-butyl hydroperoxide was measured.
  • The effects of ascorbate, bioflavonoids (epicatechin, quercetin, rutin), and Trolox were assessed, alone and in combination.

    • Main Results:

    • Ascorbate effectively protected cytochrome c in aqueous solution but failed in liposomes.
    • Bioflavonoids epicatechin and quercetin, similar to TMPD, restored ascorbate's protective function in liposomes.
    • Quercetin synergistically enhanced ascorbate's protection, while rutin and Trolox were less effective.

    Conclusions:

    • Bioflavonoids are crucial for mediating ascorbate's electron transfer into hydrophobic membrane environments.
    • Certain bioflavonoids are essential for the antioxidant protection of cytochrome c within biological membranes.
    • This highlights a potential role for bioflavonoids in modulating cytochrome c's hydroperoxidase activity and overall cellular redox balance.