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limonoids from Khaya senegalensis.

Sami A. Khalid1, Gerda M. Friedrichsen, Arsalan Kharazmi

  • 1Department of Pharmacognosy, Faculty of Pharmacy, University of Khartoum, P.O. Box 1996, Khartoum, Sudan

Phytochemistry
|November 17, 2001
PubMed
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Researchers identified a new limonoid, 2,6-dihydroxyfissinolide, from Khaya senegalensis bark. While showing moderate antiprotozoal activity, spectral assignments for related compounds were also clarified.

Area of Science:

  • Natural Product Chemistry
  • Organic Chemistry
  • Pharmacognosy

Background:

  • Khaya senegalensis is a tree species known for its rich phytochemical profile.
  • Limonoids are a class of terpenoids found in plants, often exhibiting diverse biological activities.
  • Previous research has explored the chemical constituents and medicinal properties of Khaya species.

Purpose of the Study:

  • To isolate and characterize new chemical compounds from the bark of Khaya senegalensis.
  • To elucidate the structures of isolated limonoids using spectroscopic methods.
  • To evaluate the antiprotozoal potential of the identified limonoids.

Main Methods:

  • Isolation of compounds from Khaya senegalensis bark using chromatographic techniques.
  • Structure elucidation employing Nuclear Magnetic Resonance (NMR) spectroscopy, including (13)C and (1)H NMR.

Related Experiment Videos

  • Assessment of antiprotozoal activity through in vitro assays.

    • Main Results:

    • A novel limonoid, 2,6-dihydroxyfissinolide, was successfully isolated and identified.
    • Re-assignment of specific signals in the (13)C NMR spectrum of fissinolide was performed.
    • Complete assignment of (1)H NMR signals for methyl 3beta-acetoxy-6-hydroxy-1-oxomeliac-14-enoate was achieved.
    • The isolated limonoids demonstrated moderate activity against protozoal parasites.

    Conclusions:

    • The study expands the knowledge of limonoids present in Khaya senegalensis.
    • Structural clarification of related limonoids was achieved through detailed spectroscopic analysis.
    • The findings suggest potential, albeit moderate, therapeutic applications for these compounds in treating protozoal infections.