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Related Experiment Videos

The Ded1 DEAD box helicase interacts with Chk1 and Cdc2.

Hsing-Yin Liu1, Bradley S Nefsky, Nancy C Walworth

  • 1Department of Pharmacology, Robert Wood Johnson Medical School, University of Medicine and Dentistry of New Jersey, Piscataway, New Jersey 08854, USA.

The Journal of Biological Chemistry
|November 17, 2001
PubMed
Summary

Ded1, a fission yeast DEAD box protein, responds to cellular stress. It interacts with Chk1 and may influence Cdc2 activity, suggesting a role in stress response pathways.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Ded1 is a DEAD box protein in fission yeast involved in translation.
  • Cyclin-dependent kinase Cdc2 is crucial for cell cycle progression.
  • Chk1 is a checkpoint protein kinase regulating cell cycle arrest.

Purpose of the Study:

  • To investigate the role of Ded1 in cellular stress responses.
  • To identify proteins interacting with Ded1.
  • To understand Ded1's potential influence on Cdc2 activity.

Main Methods:

  • Screening for multi-copy suppressors of a cold-sensitive cdc2 mutant.
  • Yeast two-hybrid screening to identify protein interactions.
  • Analysis of Ded1 polypeptide modification under stress conditions.

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Main Results:

  • Ded1 was identified as a multi-copy suppressor of a cdc2 mutant.
  • Ded1 physically interacts with the checkpoint kinase Chk1.
  • Ded1 undergoes distinct modifications in response to heat shock and carbon source depletion.

Conclusions:

  • Ded1 plays a role in cellular stress response pathways in fission yeast.
  • The interaction between Ded1 and Chk1 suggests a functional link between translation and cell cycle control.
  • Differential modification of Ded1 under stress indicates a regulatory mechanism impacting its function.