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Double-stranded RNA-mediated interference with plant virus infection.

F Tenllado1, J R Díaz-Ruíz

  • 1Departamento de Biología de Plantas, Centro de Investigaciones Biológicas, CSIC, Velázquez 144, 28006 Madrid, Spain.

Journal of Virology
|November 17, 2001
PubMed
Summary

Double-stranded RNA (dsRNA) effectively inhibits plant virus infections by triggering sequence-specific RNA interference. This method, utilizing viral dsRNA delivered to plant cells, offers a reliable strategy against tobamoviruses, potyviruses, and alfamoviruses.

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Area of Science:

  • Plant pathology
  • Molecular biology
  • Virology

Background:

  • Double-stranded RNA (dsRNA) is a known inducer of interference phenomena in plants and animals.
  • RNA interference (RNAi) is a conserved biological process involving sequence-specific silencing of gene expression.

Purpose of the Study:

  • To investigate the potential of externally applied dsRNA in interfering with plant virus infections.
  • To demonstrate the sequence-specific and dose-dependent nature of dsRNA-mediated interference in plants.

Main Methods:

  • Mechanical inoculation of plant leaves with viral dsRNA.
  • Agrobacterium-mediated transient expression assay for dsRNA delivery.
  • Assessing interference against tobamovirus, potyvirus, and alfamovirus infections.

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Main Results:

  • Successful interference with infections by three distinct plant virus groups using sequence-specific dsRNA.
  • Demonstrated that interference is triggered by dsRNA, not single-stranded RNA.
  • Observed a dose-dependent relationship between dsRNA application and the level of interference.

Conclusions:

  • External application of viral dsRNA can induce sequence-specific interference with plant virus infections.
  • The mechanism resembles RNA interference observed in animals and is linked to posttranscriptional gene silencing (PTGS).
  • dsRNA acts as an efficient initiator of PTGS, targeting viral RNA for degradation during natural infections.