Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Steel factor regulates cell cycle asymmetry.

C Mantel1, P Hendrie, H E Broxmeyer

  • 1Department of Microbiology, Walther Oncology Center, Indiana University School of Medicine, 1044 West Walnut Street, Indianapolis, IN 46202-5121, USA. cmantel@iupui.edu

Stem Cells (Dayton, Ohio)
|November 20, 2001
PubMed
Summary

Steel factor (SLF) synergy with GM-CSF in MO7e cells links proliferation asymmetry to rapid progenitor expansion. This suggests SLF regulates temporal asymmetry and cell cycle heterogeneity, potentially involving p27kip-1.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Impact of electronic immunization registries and electronic logistics management information systems in four low-and middle-income countries: Guinea, Honduras, Rwanda, and Tanzania.

Vaccine·2025
Same author

Vaccine implementation factors affecting maternal tetanus immunization in low- and middle-income countries: Results of the Maternal Immunization and Antenatal Care Situational Analysis (MIACSA) project.

Vaccine·2020
Same author

Resistance training in patients with schizophrenia: Concept and proof of principle trial.

Journal of psychiatric research·2019
Same author

Is there a need for morphologic exam to detect relapse in AML if multi-parameter flow cytometry is employed?

Leukemia·2017
Same author

DPP4 truncated GM-CSF and IL-3 manifest distinct receptor-binding and regulatory functions compared with their full-length forms.

Leukemia·2017
Same author

Small-molecule inhibitors targeting the DNA-binding domain of STAT3 suppress tumor growth, metastasis and STAT3 target gene expression in vivo.

Oncogene·2016

Area of Science:

  • Hematopoiesis
  • Cell Biology
  • Stem Cell Biology

Background:

  • Asymmetric cell division is crucial for stem cell maintenance and progenitor differentiation in the hematopoietic system.
  • Temporal cell cycle asymmetry and heterogeneity are vital for tissue organization.
  • Hematopoietic stem cells (HSCs) are influenced by various cytokines, with some synergizing for progenitor expansion.

Purpose of the Study:

  • To investigate the link between proliferation asymmetry and Steel Factor (SLF) synergy with GM-CSF in the MO7e cell line.
  • To explore the role of SLF in regulating temporal asymmetry and cell cycle heterogeneity in vitro.
  • To identify potential regulators of this temporal asymmetry, such as p27kip-1.

Main Methods:

  • Utilized the human growth factor-dependent MO7e cell line as a model for synergistic proliferation.

Related Experiment Videos

  • Analyzed proliferation patterns and cell cycle dynamics under SLF and GM-CSF stimulation.
  • Investigated the expression or activity of cell cycle regulators like p27kip-1.

    • Main Results:

    • Demonstrated a link between proliferation asymmetry and SLF/GM-CSF synergy in MO7e cells.
    • Provided evidence that SLF can regulate temporal asymmetry and cell cycle heterogeneity in vitro.
    • Indicated that the CDK-inhibitor p27kip-1 may be involved in regulating this temporal asymmetry.
    • Proposed that SLF/GM-CSF synergy shifts proliferation from asymmetric/heterogeneous to symmetric/synchronous patterns, driving rapid expansion.

    Conclusions:

    • SLF/GM-CSF synergy in MO7e cells is associated with a shift in proliferation patterns, contributing to rapid progenitor expansion.
    • This kinetic model of asymmetry provides insights into how cytokines influence cell cycle regulation.
    • The MO7e/SCF-synergy/asymmetry model can be valuable for studying cytokine effects on cell cycle asymmetry in hematopoietic stem cells.