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Related Experiment Videos

Islet cryopreservation using intracellular preservation solutions.

J R Lakey1, R V Rajotte, C A Fedorow

  • 1Surgical-Medical Research Institute, Department of Surgery, University of Alberta, Edmonton, Canada. jonathan.lakey@ualberta.ca

Cell Transplantation
|November 21, 2001
PubMed
Summary

Cryopreservation of islets using University of Wisconsin (UW) or HypoThermosol (HTS) solutions with dimethyl sulfoxide (DMSO) significantly improves islet recovery and viability compared to standard media. These findings enhance islet transplant program flexibility.

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Area of Science:

  • Cellular Biology
  • Transplantation Immunology
  • Biomedical Engineering

Background:

  • Traditional islet cryopreservation methods lack optimization for ionic balance and oxidative stress during hypothermic storage.
  • The University of Wisconsin (UW) solution and HypoThermosol (HTS) are established preservation solutions with unique compositions.
  • Evaluating these solutions for islet cryopreservation is crucial for advancing transplant programs.

Purpose of the Study:

  • To assess the efficacy of UW solution and HTS as media for canine islet cryopreservation.
  • To compare the post-thaw recovery and functional viability of islets cryopreserved in UW, HTS, and Medium 199.

Main Methods:

  • Canine islets were cryopreserved using a standard protocol with dimethyl sulfoxide (DMSO) as a cryoprotectant.

Related Experiment Videos

  • Cryopreservation media included UW solution, HTS, and Medium 199 with or without fetal calf serum (FCS).
  • Islet recovery and glucose-stimulated insulin secretion were measured post-thaw and after 48-hour culture.

    • Main Results:

    • Islet recovery was significantly higher in groups cryopreserved with UW or HTS in combination with DMSO compared to Medium 199 alone.
    • Islets cryopreserved without DMSO showed minimal recovery (<15%).
    • Functional viability, assessed by insulin secretion, was highest in islets preserved with UW and HTS solutions.

    Conclusions:

    • Intracellular-based preservation solutions like UW and HTS, when used with DMSO, enhance postcryopreservation islet survival.
    • These findings support the use of UW and HTS for improved islet cryopreservation, increasing transplant program flexibility.
    • Optimized cryopreservation media are essential for successful islet transplantation.