Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Calculated background elimination in quantifying nitric-oxide synthase enzyme activity.

F Stonek1, W Tschugguel, J C Huber

  • 1Department of Gynecology and Obstetrics, Division of Gynecological Endocrinology and Reproductive Medicine, University Hospital Vienna, Waehringer Guertel 18-20 EBO5Q, A-1090, Vienna, Austria. felix.stonek@akh-wien.ac.at

Journal of Biochemical and Biophysical Methods
|November 21, 2001
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Feasibility of the WHO Infection Prevention and Control Assessment Framework in long-term care facilities: a pilot study in the Netherlands.

Antimicrobial resistance and infection control·2026
Same author

Candida auris in Dutch hospitals: are we ready for it?

The Journal of hospital infection·2024
Same author

Sensitivity of C-Reactive Protein and Procalcitonin Measured by Point-of-Care Tests to Diagnose Urinary Tract Infections in Nursing Home Residents: A Cross-Sectional Study.

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America·2020
Same author

"A false sense of confidence" The perceived role of inflammatory point-of-care testing in managing urinary tract infections in Dutch nursing homes: a qualitative study.

BMC geriatrics·2020
Same author

Progesterone for prevention of preterm birth in women with short cervical length: 2-year infant outcomes.

Ultrasound in obstetrics & gynecology : the official journal of the International Society of Ultrasound in Obstetrics and Gynecology·2020
Same author

Polymorphism 1558 C > T in the aromatase gene (CYP19A1) in low-grade endometrial stromal sarcoma.

European journal of gynaecological oncology·2012

This study introduces a mathematical method to accurately quantify low nitric-oxide synthase (NOS) activity by correcting for background noise in the standard citrulline assay. This improves the reliability of NOS activity measurements in low-protein samples.

Area of Science:

  • Biochemistry
  • Enzymology
  • Assay Development

Background:

  • The standard assay for nitric-oxide synthase (NOS) activity measures L-arginine to L-citrulline conversion.
  • Low protein samples (< 25 microg/microl) present challenges in NOS activity assays due to indistinguishable sample and blank values.
  • Radioactive-labeled arginine in the eluate contributes to background noise, complicating accurate quantification.

Purpose of the Study:

  • To develop a simple, mathematical method for quantifying low NOS activity.
  • To address the issue of background noise in NOS activity assays with low protein amounts.
  • To improve the accuracy and reliability of the L-citrulline assay for NOS activity.

Main Methods:

  • A mathematical approach was employed to determine and remove background values from assay results.

Related Experiment Videos

  • The method focuses on quantifying low nitric-oxide synthase (NOS) activity.
  • The technique was applied to samples with low protein concentrations (< 25 microg/microl).

    • Main Results:

    • The developed method effectively quantifies low NOS activity by mathematically removing background noise.
    • It allows for better differentiation between sample and blank values in low-protein samples.
    • The mathematical correction improves the accuracy of the citrulline assay for NOS activity.

    Conclusions:

    • A simple mathematical method can accurately quantify low nitric-oxide synthase (NOS) activity.
    • This approach enhances the standard citrulline assay, particularly for samples with low protein content.
    • The method offers an improvement for reliable NOS activity measurement in challenging biological samples.