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Cellular solid-phase binding assay and mass spectrometry for screening of alpha 4 beta 7 integrin antagonists
D Gottschling1, J Boer, A Schuster
1Institut für Organische Chemie und Biochemie, Technische Universität München, Lichtenbergstrasse 4, D-85747 Garching, Germany.
Bioorganic & Medicinal Chemistry Letters
|November 21, 2001
Abstract:
A qualitative cellular solid-phase binding assay for screening alpha 4 beta 7 integrin antagonists attached via photolinker to TentaGel Macrobeads has been developed. An activation of the integrins with Mn(2+) was necessary to achieve binding to the bead bound antagonists. The identification of the resin bound compounds was done by mass spectrometry.