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Updated: Oct 3, 2026

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
Published on: April 28, 2021
[Human hR24L gene is involved in DNA excision repair and recombination repair]
1Laboratory of Molecular Biology, Laboratory of Cancer and Radiation Diseases, Third Hospital of Beijing Medical University, Beijing 100083.
Objective:
To study the correction phenotype of temperature sensitive mutant rad24LR of S. cerevisiae and to clone wild human functional homologue.
Methods:
The temperature sensitive mutant rad24LR was studied by complementation test. Transformation of rad24LR was performed with human cDNA expression library to screen for reversion phenotype.
Results:
RAD24L can complement the radiation sensitive phenotype of the mutation. Its homologue of human cDNA was cloned by screening transformed rad24LR with human expression library for reversion phenotype.
Conclusion:
Human DNA repair gene hR24L was successfully cloned in this study. The product hR24L is involved in both DNA excision repair and recombination repair.
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