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Updated: Oct 3, 2026
Yeast As a Chassis for Developing Functional Assays to Study Human P53
Published on: August 4, 2019
[Tumour suppressor p53 gene sequence mutations in pleomorphic adenoma of salivary gland]
Objective:
To detect p53 exon 7 and 8 mutation and to study genesis and biological characteristics of pleomorphic adenoma of salivary galand.
Methods:
Polymerase chain reaction- single-strand conformational polymorphism (PCR-SSCP) and DNA sequencing analysis were used.
Results:
PCR-SSCP analysis showed that in 11 patients with positive SSCP, 7 showed abnormal exon 7 and 3 abnormal exon 8. Comparison of SSCP results with DNA sequencing showed that patients all patients with p53 exon 7 and 8 mutations were SSCP positive. Exon 7 mutation pattern was point mutation(T-->C). Exon 8 mutation pattern included base substitution (point mutation) (C-->A) and frame-shiftmutation (base insertion and base loss).
Conclusions:
Polycodon and poly-pattern mutations appeared in p53 exon 7 and 8. Pleomorphic adenoma of salivary galand was similar to malignant tumor in p53 gene mutation. Poly-codon mutation could occur in the same case.
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