Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Identification of sonic hedgehog-responsive genes using cDNA microarray.

M Kato1, N Seki, S Sugano

  • 1Division of Signal Transduction, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-01, Japan.

Biochemical and Biophysical Research Communications
|November 22, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Production of phytochelatins in Polygonum cuspidatum on exposure to copper but not to zinc.

Journal of pharmacobio-dynamics·1992
Same author

[A case report of pulmonary embolectomy for acute pulmonary embolism].

Kyobu geka. The Japanese journal of thoracic surgery·1992
Same author

Transient bullous dermolysis of the newborn. Retention of anchoring fibril- and basal lamina-like structures in keratinocytes and evidence of collagenolysis.

Journal of cutaneous pathology·1992
Same author

Activation of phospholipases in platelets by polyclonal antibodies against a surface membrane protein.

Biochimica et biophysica acta·1992
Same author

The growth regulation of keratinocytes.

The Journal of dermatology·1992
Same author

The role of growth factor in wound healing.

The Journal of dermatology·1992

Sonic hedgehog (Shh) signaling is crucial in development and disease. This study identified two new Shh-responsive genes, Ceruloplasmin (Cp) and inter-alpha-trypsine inhibitor heavy chain H3 (ITIH3), using microarray analysis.

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genetics

Background:

  • Sonic hedgehog (Shh) is a critical secreted signaling protein involved in development and disease.
  • Understanding downstream molecules of Shh signaling is essential for further research.

Purpose of the Study:

  • To identify novel Shh-responsive genes using a cDNA microarray approach.
  • To investigate the regulatory pathways of newly identified Shh-responsive genes.

Main Methods:

  • Utilized an in-house cDNA microarray to screen for Shh-responsive genes.
  • Employed a neuroepithelial cell line (MNS-70) for gene expression analysis.
  • Investigated gene regulation by Gli transcription factors and developmental expression patterns.

Related Experiment Videos

Main Results:

  • Identified two novel Shh-responsive genes: Ceruloplasmin (Cp) and inter-alpha-trypsine inhibitor heavy chain H3 (ITIH3).
  • ITIH3 expression was found to be regulated by Gli transcription factors downstream of Shh.
  • Cp expression appeared to be regulated by Gli-independent pathways, with its mRNA detected in the developing mouse brain adjacent to Shh expression.

Conclusions:

  • Microarray technology is an effective tool for discovering developmentally regulated genes.
  • Cp and ITIH3 are newly identified Shh-responsive genes with distinct regulatory mechanisms.
  • This research expands the understanding of molecular pathways downstream of Sonic hedgehog signaling.