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Related Experiment Videos

Disabled-1 interacts with a novel developmentally regulated protocadherin.

R Homayouni1, D S Rice, T Curran

  • 1Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, Tennessee 38105, USA.

Biochemical and Biophysical Research Communications
|November 22, 2001
PubMed
Summary

Researchers identified a novel protocadherin, mouse Pcdh18, that interacts with Disabled-1 (Dab1). This interaction suggests mPcdh18 may play a role in Reelin signaling and mammalian brain development.

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Area of Science:

  • Neuroscience
  • Molecular Biology
  • Genetics

Background:

  • Disabled-1 (Dab1) is crucial for Reelin signaling, mediating neuronal migration and positioning in mammalian brain development.
  • Understanding the Reelin-Dab1 pathway requires identifying interacting proteins.

Purpose of the Study:

  • To identify novel proteins interacting with Dab1.
  • To characterize a newly identified Dab1-interacting protein and its potential role in brain development.

Main Methods:

  • Yeast two-hybrid screening to identify Dab1-interacting proteins.
  • Molecular characterization of the identified protocadherin, mouse Pcdh18 (mPcdh18).
  • Expression analysis of mPcdh18 in embryonic and adult mouse tissues, including the brain.

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Main Results:

  • A novel protocadherin, mPcdh18, was identified as a binding partner for the Dab1 phosphotyrosine binding (PTB) domain.
  • mPcdh18 shares structural features with other protocadherins and interacts with Dab1 via its C-terminal region.
  • mPcdh18 exhibits dynamic temporal and spatial expression in the embryonic brain and is also found in adult lung and kidney.

Conclusions:

  • mPcdh18 is a component of signaling pathways involving PTB-containing proteins.
  • The findings suggest a potential role for mPcdh18 in mammalian brain development through interaction with the Reelin-Dab1 pathway.