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Characterization of recombinant Desulfovibrio gigas ferredoxin
P Rodrigues1, F Graça, A L Macedo
1Departamento de Química, Centro Quìmica Fina e Biotecnologia, Faculdade de Ciências e Tecnologia, Universidade Nova de Lisboa, 2825 Monte de Caparica, Portugal.
Biochemical and Biophysical Research Communications
|November 22, 2001
Abstract:
Dg ferredoxin gene was cloned using the polymerase chain reaction (PCR), inserted into vector pT7-7, and overexpressed in Escherichia coli (E. coli) grown in aerobic media. The recombinant protein is a dimer and contains a [3Fe-4S] cluster per monomer. EPR and (1)H NMR data of recombinant and wild-type protein are compared.