Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Reelin does not directly influence axonal growth.

Y Jossin1, A M Goffinet

  • 1Neurobiology Unit, University of Namur School of Medicine, B5000 Namur, Belgium.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|November 22, 2001
PubMed
Summary

Reelin, a key protein in brain development, does not directly guide cortical axon growth. Its observed effects on axonal pathways appear indirect, stemming from Reelin deficiency-induced architectural changes.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Neural progenitor fate decision defects, cortical hypoplasia and behavioral impairment in Celsr1-deficient mice.

Molecular psychiatry·2017
Same author

Reelin receptors in developing laminated brain structures of mouse and human.

The European journal of neuroscience·2004
Same author

Reelin expression during embryonic brain development in Crocodylus niloticus.

The Journal of comparative neurology·2003
Same author

The role of reelin in the development and evolution of the cerebral cortex.

Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas·2002
Same author

Expression of the ankyrin repeat domain 6 gene (Ankrd6) during mouse brain development.

Developmental dynamics : an official publication of the American Association of Anatomists·2002
Same author

Developmental expression profiles of Celsr (Flamingo) genes in the mouse.

Mechanisms of development·2002

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Molecular Biology

Background:

  • Reelin is a glycoprotein crucial for brain development, particularly cortical and hippocampal architectonics.
  • It is synthesized by Cajal-Retzius cells and influences axonal pathways in the hippocampus.
  • Reelin undergoes proteolytic processing in vivo.

Purpose of the Study:

  • To investigate the direct effects of Reelin on axonal elongation.
  • To differentiate between processed and full-length Reelin's impact on axon growth.
  • To assess Reelin's role in cortical axon guidance.

Main Methods:

  • Three-dimensional collagen gel assay used for measuring axonal elongation from reeler cortical explants.
  • Sources of Reelin included normal explants (processed) and Reelin-transfected HEK293T cells (full-length).

Related Experiment Videos

  • Semaphorin 3F was used to validate assay reliability through its repulsive action.

    • Main Results:

    • Neither full-length nor processed Reelin demonstrated significant attraction or repulsion of cortical axons.
    • The assay's reliability was confirmed by semaphorin 3F's significant repulsive effect (p < 0.0001).
    • No direct influence of Reelin on axonal growth was detected.

    Conclusions:

    • The study suggests Reelin's effects on axonal pathways are indirect, likely secondary to Reelin deficiency-induced architectonic disturbances.
    • Cajal-Retzius cells' influence on neural connectivity may be largely independent of Reelin.
    • Reelin's primary role might be in establishing brain architecture rather than direct axon guidance.