Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structure of Ca(2+)-loaded human grancalcin.

J Jia1, N Borregaard, K Lollike

  • 1Biotechnology Research Institute, National Research Council of Canada, 6100 Royalmount Avenue, Montreal, Quebec H4P 2R2, Canada.

Acta Crystallographica. Section D, Biological Crystallography
|November 22, 2001
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Up-regulation of miR-934 serves as an independent prognostic factor for lung cancer and promotes proliferation, migration and invasion of non-small cell lung cancer cells.

Journal of biological regulators and homeostatic agents·2021
Same author

Observation and Measurement of Forward Proton Scattering in Association with Lepton Pairs Produced via the Photon Fusion Mechanism at ATLAS.

Physical review letters·2021
Same author

Search for Heavy Resonances Decaying into a Photon and a Hadronically Decaying Higgs Boson in pp Collisions at sqrt[s]=13  TeV with the ATLAS Detector.

Physical review letters·2021
Same author

Search for Higgs Boson Decays into a Z Boson and a Light Hadronically Decaying Resonance Using 13 TeV pp Collision Data from the ATLAS Detector.

Physical review letters·2020
Same author

The effect of <i>Clostridium tyrobutyricum Spo0A</i> overexpression in the intestine of mice.

Beneficial microbes·2020
Same author

Dijet Resonance Search with Weak Supervision Using sqrt[s]=13  TeV pp Collisions in the ATLAS Detector.

Physical review letters·2020

Grancalcin, a cytosolic calcium-binding protein, shows minor structural changes upon Ca(2+) binding. Calcium is necessary but not sufficient for significant conformational changes in PEF proteins.

Area of Science:

  • Biochemistry
  • Structural Biology
  • Molecular Biology

Background:

  • Grancalcin is a cytosolic Ca(2+)-binding protein belonging to the PEF protein family, characterized by five EF-hand motifs.
  • The N-terminus of grancalcin contains a disordered segment, and its fifth EF-hand facilitates dimerization.
  • Previous studies determined the structure of full-length grancalcin but noted disorder in the N-terminal region.

Purpose of the Study:

  • To investigate the structure of a truncated grancalcin lacking N-terminal residues.
  • To elucidate the conformational changes induced by Ca(2+) binding in grancalcin.
  • To test the hypothesis regarding calcium's role in PEF protein conformational changes.

Main Methods:

  • X-ray crystallography was used to determine the structure of truncated grancalcin.

Related Experiment Videos

  • Structures were obtained in both the absence (apo form) and presence of Ca(2+).
  • Comparative analysis with homologous proteins like ALG-2 was performed.

    • Main Results:

    • The structure of truncated grancalcin was determined in apo and Ca(2+)-bound states.
    • In the Ca(2+)-bound form, ions were located in the EF1 and EF3 hands.
    • Ca(2+) binding induced only minor conformational changes, primarily in the EF1 loop.

    Conclusions:

    • Calcium binding to grancalcin results in minimal structural alterations.
    • This supports the idea that Ca(2+) is essential but insufficient for significant conformational changes in PEF proteins.
    • Differences in EF-hand orientation between grancalcin and ALG-2 may influence calcium-induced conformational changes.