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[Solid phase radioimmunoassay for plasma testosterone using plastic microtiter tray (author's transl)]
Nihon Naibunpi Gakkai Zasshi
|March 20, 1975
Summary
This study introduces a simplified solid-phase radioimmunoassay for plasma testosterone, eliminating chromatography and enabling simultaneous measurement of numerous samples. The method is cost-effective and suitable for routine clinical use, offering accurate testosterone level determination.
Area of Science:
- Endocrinology
- Immunochemistry
- Analytical Chemistry
Context:
- Radioimmunoassay (RIA) is a common method for measuring hormone levels.
- Traditional RIA methods can be complex and time-consuming, often requiring chromatography.
- Simultaneous measurement of multiple samples is desirable for efficiency.
Purpose:
- To develop a simplified solid-phase radioimmunoassay for plasma testosterone.
- To eliminate the need for chromatography in testosterone measurement.
- To enable high-throughput analysis of plasma testosterone levels.
Summary:
- A solid-phase RIA using a disposable microtiter tray (DMT) was developed for plasma testosterone measurement.
- The method involves antibody coating of DMT, incubation with plasma samples and 3H-testosterone, followed by radioactivity counting.
- The assay demonstrated good sensitivity (10 pg/tube), recovery (99%), and precision (CV < 11.2%), with strong correlation (r=0.938) to existing methods.
Impact:
- This simplified RIA method is economically feasible and efficient for routine clinical use.
- It allows for simultaneous measurement of many samples, reducing time and labor.
- Accurate determination of plasma testosterone levels supports clinical diagnosis and monitoring of various endocrine conditions.