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[Effect of basic fibroblast growth factor on cultured spiral ganglion cells following glutamate treatment]
Objective:
To understand the effects of basic fibroblast growth factor (bFGF) on survival and neurogenesis of the cultured spiral ganglion cell (SGC) of mouse after exposed to glutamate, and the effects of hFGF and glutamate on free intracellular calcium ion concentration.
Methods:
After exposed to 2 x 10(-2) mol/L glutamate for 2 hours, the medium of SGC was replaced with the media containing 25, 50, 100 micrograms/L bFGF. Medium without bFGF was used as control.
Results:
Twenty-four hours later, more cells in the control group showed signs of cell death than in bFGF groups. After 14 days, specimens were fixed in 4% paraformaldehyde and stained with toluidine blue. It was shown that the SGC in medium with bFGF exhibited more survival and longer neurites, and the effect was dose dependent (P < 0.01). Application of glutamate to the medium induced an increase to the the free intracellular calcium ion concentration in SGC, but bFGF had no such effect.
Conclusion:
Glutamate excitotoxicity was associated with free intracellular calcium ion concentration elevation in SGC, and bFGF could protect SGC against glutamate neurotoxicity.
