[Regulating function of enzymization and deenzymization of the lactate dehydrogenase isozymes in the mouse tissues...

Study the characteristics of changes of LDH enzyme map of mouse under slight hypoxia.

Treated mouse with artificial hypoxia, various tissue biopsies were made for the test of LDH enzymatic activity by specific staining technical LDH(1-5)relative percent enzymatic activity were measured with CS-910 dual-wavelength thin-layer chromatography scanner.

The relative percent enzymatic activity of LDH isozymes of various tissues after slight hypoxia shift to the isozymes LDH1 and LDH2, whose principal subunits are H subunits, and the relative percent enzymatic activity of LDH1 (H4), LDH2-(H3M) increased (the relative percent enzymatic activity of LDH2 increased markedly, P < 0.05), while LDH (M4) in various tissues decreased prominently except the cardiac muscle, and that of LDH4 (HM3) decreased as well. After the PAGE of the hypoxia treated cardiac muscle specimen was made, activity subbands originated regularly in the isozyme pattern of LDH, with the regularity of LDH1 (0 subband), LDH2 (0-1 subbands), LDH3 (0-2 subbands), LDH4 (1-3 subbands), LDH5 (2-4 subbands). After adding appropriate amount of NAD+ to mouse cardiac muscle specimen with hypoxia, PAGE showed the numbers of subbands of four kinds of isozymes (LDH2-LDH5) decreased even totally disappeared in the isozyme pattern.

The negative feedback regulation of enzymization and deenzymization of LDH isozymes is one of mouse stress responses to slight hypoxia.