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Interaction between gene p53 and oncogene mdm2 in human glandular lung cancer cell line GLC-82
1Department of Molecular Biology, Xijing Hospital, 4th Military Medical University, Xi'an 710032, China.
Objective:
To study the interaction between oncogene mdm2 and wp53 in human glandular lung cancer cell line GLC-82.
Methods:
By lipofectamine-mediated DNA transfection, wp53 and mdm2 were transfected separately or co-transfected into GLC-82 cells via retrovival vector pDOR-neo, a carrier of wp53 and mdm2.
Results:
The growth of GLC-82 cells was blocked and their DNA synthesis inhibited by wp53, its colony-forming rate in soft agar culture and the tumorigenicity in nude mice declined and mdm2 antagonized the function of wp53.
Conclusion:
After the recombinant vector pDOR-mdm2 was transfected into GLC-82 cells containing wp53, mdm2 partially deprives wp53 of its function of inhibiting the growth of GLC-82 cells.