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Determination of astragaloside IV in Radix astragali (Astragalus membranaceus var. monghulicus) using
1Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, University of Illinois at Chicago, 60612, USA.
Abstract:
A reverse-phase high-performance liquid chromatographic method is developed for the determination of astragaloside IV, a characteristic constituent in Radix Astragali. Samples are analyzed by means of a reverse-phase column (Zorbax Eclipse XDB C18) using acetonitrile and water under gradient conditions as the mobile phase for 30 min. An evaporative light-scattering detector is used and set at an evaporating temperature of 43 degrees C with a nebulizing gas (compressed air) pressure of 3.4 bar. The detection limit (signal-to-noise ratio > 5) of astragaloside IV is 40 ng on-column.
