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Enterotoxins of Staphylococcus aureus induce a cytopathic effect in cell lines
G Normanno1, G Celano, A Dambrosio
1Department of Health and Animal Welfare--Faculty of Veterinary Medicine--Valenzano (Bari) Italy.
The New Microbiologica
|November 23, 2001
Abstract:
We describe the use of cell cultures for the detection of Staphylococcus aureus enterotoxins. Madin Darby Bovine Kidney (MDBK), Bovine Embryo lung cells (PEB) and Dog Carcinoma Cell line (A-72) cell monolayers were tested. MDBK, A-72 and PEB cell lines proved to be susceptible to the cytotoxic effect induced by staphylococcal enterotoxins. A cytopathic effect was observed in some cases after 2 hours of incubation. The PEB cell line was the most susceptible and may be used as a useful and cheap method for screening enterotoxigenic S. aureus strains.