Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Gene expression profiling of aging using DNA microarrays.

Richard Weindruch1, Tsuyoshi Kayo, Cheol Koo Lee

  • 1Department of Medicine, University of Wisconsin-Madison and Veterans Administration Hospital, Geriatric Research, Education and Clinical Center, Madison, WI 53705, USA.

Mechanisms of Ageing and Development
|November 24, 2001
PubMed
Summary

Gene expression profiles reveal aging changes in mouse muscle and brain. Caloric restriction can prevent these age-related molecular alterations, suggesting a method to assess biological age and intervention effectiveness.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Dietary restriction in aging and longevity.

Nature aging·2026
Same author

Somatostatin Receptor Type 2 and Thyroid-Stimulating Hormone Receptor Expression in Oncocytic Thyroid Neoplasms: Implications for Prognosis and Treatment.

Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc·2023
Same author

Living high - training low model applied to C57BL/6J mice: Effects on physiological parameters related to aerobic fitness and acid-base balance.

Life sciences·2023
Same author

SIRT3 deficiency decreases oxidative metabolism capacity but increases lifespan in male mice under caloric restriction.

Aging cell·2022
Same author

Aerobic training associated with an active lifestyle exerts a protective effect against oxidative damage in hypothalamus and liver: The involvement of energy metabolism.

Brain research bulletin·2021
Same author

Retraction Note to: Exercise-induced mitochondrial p53 repairs mtDNA mutations in mutator mice.

Skeletal muscle·2021

Area of Science:

  • Molecular biology
  • Gerontology
  • Genomics

Background:

  • Aging is a complex process affecting various tissues.
  • Gene expression changes are associated with aging in organs like muscle and brain.
  • Caloric restriction is known to influence aging and lifespan.

Purpose of the Study:

  • To profile gene expression during aging in mouse skeletal muscle and brain.
  • To investigate the impact of caloric restriction on age-related gene expression changes.
  • To explore DNA microarrays as a tool for measuring biological age and intervention efficacy.

Main Methods:

  • Utilized high-density oligonucleotide arrays to analyze gene expression.
  • Examined gene expression profiles in gastrocnemius muscle, cerebellum, and neocortex of aging male C57BL/6 mice.

Related Experiment Videos

  • Compared gene expression patterns between normally aging mice and those subjected to caloric restriction.

    • Main Results:

    • Identified distinct gene expression profiles associated with aging in skeletal muscle and brain.
    • Demonstrated that caloric restriction can prevent or slow down the establishment of these age-related gene expression alterations.
    • Highlighted tissue-specific gene expression patterns linked to the aging process.

    Conclusions:

    • Gene expression profiling using DNA microarrays can characterize the aging process in a tissue-specific manner.
    • Caloric restriction effectively modulates molecular aging markers at the gene expression level.
    • DNA microarrays offer a potential tool for assessing biological age and evaluating anti-aging interventions at a molecular level.