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Published on: May 27, 2016
Molecular cloning of a murine type III sodium-dependent phosphate cotransporter (Pit-2) gene promoter
L Bai1, J F Collins, H Xu
1Department of Pediatrics and Physiology, Steele Memorial Children's Research Center, University of Arizona Health Sciences Center, 1501 North Campbell Avenue, Tucson, AZ 85724, USA.
Abstract:
We report the novel cloning and preliminary characterization of a murine type III sodium-dependent phosphate cotransporter (Pit-2) gene promoter. Five promoter/luciferase reporter gene constructs, -1816/+61, -1620/+61, -1223/+61, -600/+61 and -225/+61, showed significant luciferase activity (6-14-fold over background) when transfected into human colon carcinoma (Caco-2) and opossum kidney (OKP) cells.
