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Biochemical studies on bovine adenovirus type 3. II. Incomplete virus
Abstract:
Incomplete virus of oncogenic bovine adenovirus type 3 (BAV3) was highly purified and its biological activity was studied. The production of incomplete virus was found to increase with a high multiplicity of infection and with a large amount of arginine in the growth medium. On infection of contact-inhibited mouse cells, incomplete virus induced cellular DNA synthesis and focus formation. Moreover, this virus was oncogenic to newborn hamsters. On infection of calf kidney cells, a permissive cell line, viral early and late RNA, viral DNA, and almost all the viral late proteins were produced, but no mature progeny virus was detected. It is, therefore, suggested that incomplete virus of BAV3 may be unable to synthesize a protein(s) (perhaps a kind of maturation protein[s]) essential for assembly of viral macromolecules for maturation.
Insights
Incomplete bovine adenovirus type 3 (BAV3) was purified and found to be oncogenic. This incomplete virus induces DNA synthesis but fails to produce mature progeny, suggesting a defect in viral maturation.
Area of Science:
- Virology
- Oncology
- Molecular Biology
Background:
- Bovine adenovirus type 3 (BAV3) is an oncogenic virus.
- Understanding the replication and assembly of BAV3 is crucial for virology and oncology research.
- The study focuses on an incomplete form of BAV3, exploring its biological activity and potential role in oncogenesis.
Purpose of the Study:
- To highly purify and characterize the biological activity of incomplete bovine adenovirus type 3 (BAV3).
- To investigate the factors influencing the production of incomplete BAV3.
- To determine the oncogenic potential and replication capabilities of incomplete BAV3 in various cell types and animal models.
Main Methods:
- High purification of incomplete BAV3.
- Infection of contact-inhibited mouse cells and calf kidney cells.
- Analysis of cellular DNA synthesis and focus formation.
- Oncogenicity testing in newborn hamsters.
- Detection of viral RNA, DNA, and proteins in infected cells.
Main Results:
- Incomplete BAV3 production increased with high multiplicity of infection and arginine levels.
- Infection of mouse cells induced cellular DNA synthesis and focus formation.
- Incomplete BAV3 demonstrated oncogenicity in newborn hamsters.
- Permissive calf kidney cells produced viral RNA, DNA, and late proteins, but no mature progeny virus.
Conclusions:
- Incomplete BAV3 is biologically active and oncogenic.
- The virus appears to be defective in the final stages of viral assembly and maturation.
- A potential deficiency in synthesizing essential maturation protein(s) is suggested as the cause for the lack of mature progeny virus.