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Carbenoxolone, a new inducer of heat shock protein 70

S Nagayama1, H Jono, H Suzaki

  • 1Department of Pharmacological Sciences, Faculty of Pharmaceutical Sciences, Kumamoto University, Japan.

Life Sciences
|November 27, 2001
PubMed

Insights

Carbenoxolone, an antiulcer drug, was found to increase heat shock protein 70 (Hsp70) expression in human cells. This suggests carbenoxolone may be a potential therapeutic agent for inducing Hsp70, offering cellular protection.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Pharmacology

Background:

  • Heat shock protein 70 (Hsp70) confers cellular and tissue protection against various stressors, including heat and disease.
  • Therapeutic strategies aim to identify safe compounds that can induce Hsp70 expression in patients.

Purpose of the Study:

  • To investigate whether carbenoxolone, an established antiulcer drug, can induce Hsp70 expression in human cell lines.
  • To explore the potential of carbenoxolone as a therapeutic agent for enhancing cellular protection via Hsp70 induction.

Main Methods:

  • Human cell lines were treated with carbenoxolone.
  • Hsp70 protein and mRNA levels were quantified.
  • Hsp70 promoter activity was assessed.

Main Results:

  • Carbenoxolone treatment led to a significant increase in Hsp70 protein expression.
  • Elevated levels of Hsp70 mRNA were observed following carbenoxolone administration.
  • Carbenoxolone enhanced Hsp70 promoter activity, indicating transcriptional activation.

Conclusions:

  • Carbenoxolone effectively induces Hsp70 expression at both protein and mRNA levels in human cells.
  • The findings suggest that carbenoxolone's ability to activate the Hsp70 promoter may contribute to its cytoprotective effects.
  • Carbenoxolone represents a potential therapeutic candidate for conditions where Hsp70 induction is beneficial.

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