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[A comparison of serological and high resolution DNA methods for HLA-B40 cross-reactive groups typing]
Objective:
To compare the high-resolution DNA typing method with serological method for HLA-B40 cross-reactive groups (CREG) typing in Chinese population.
Methods:
A total of 199 consecutive samples were entered into the study, including 177 identified serologically as B40 CREG positive, 12 ambiguous and 10 negative controls. Double-blind typing for HLA-B40 CREG was carried out using PCR-SSP high-resolution DNA typing and serological one-step monoclonal antibody technique, respectively.
Results:
In 3 of the samples serological typing could not be performed owing to poor cell viability, while in 1 PCR-SSP typing could not be done owing to poor DNA. Among samples that were successfully typed by both methods, all alleles of HLA-B40 CREG could be accurately distinguished by PCR-SSP. It was proved that PCR-SSP was a high-sensitivity and high-specificity technique. However, serology showed 11.2% misassignments. The overall time for serological typing was 2 hours, and for DNA typing, 5 hours. The percentages of HLA-B40 CREG alleles in Chinese population were 67.4% for B60, 20.8% for B61, 9.6% for B4801 and 2.2% for B4005.
Conclusion:
High resolution PCR-SSP typing for HLA-B40 CREG could be used in routine clinical practice with a greater precision than serology.