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[Study of apoptosis induced by indomethacin in chronic myeloid leukemia]
1Laboratory of Molecular Hematology, Division of Hematology, Second Affiliated Hospital, Hunan Medical University, Changsha 410011.
Objective:
To explore the effect of indomethacin(IN) on the apoptosis of chronic myeloid leukemia(CML) cells, and its molecular mechanisms, for the purpose of screening a new antileukemic agent.
Methods:
CML cell line K562 and fresh bone marrow cells from 6 untreated Ph+ CML patients were used for in vitro culture study. The effects of IN on cells were determined by cell morphology, flow cytometry, DNA electrophoresis, and RT-PCR.
Results:
1. IN induced apoptosis of K562 and fresh CML cells and inhibited the proliferation of K562 cells. 2. A synergic effect of inducing K562 cell apoptosis was observed when IN combined with Vp16. 3. IN down-regulated the level of bcl-2 mRNA without changing the level of bax mRNA in K562 cells.
Conclusion:
IN can induce apoptosis and inhibit proliferation in CML cells, and increase the sensitivity of CML cells to Vp16. Down-regulation of bcl-2 mRNA may be one of the mechanisms of CML cell apoptosis induced by IN.