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Nitric oxide synthase/nitric oxide pathway mediates intussusception pathogenesis in rats
1Department of Pediatric Surgery and Central Laboratory, First Clinical Hospital, Beijing Medical University, Beijing 100083, China.
Chinese Medical Journal
|November 28, 2001
Summary
The nitric oxide synthase/nitric oxide (NOS/NO) pathway is crucial in intussusception (IN) development. Inhibiting NO production may offer a novel strategy for preventing IN.
Area of Science:
- Physiology
- Gastroenterology
- Pharmacology
Background:
- Intussusception (IN) is a significant gastrointestinal emergency.
- The role of the nitric oxide synthase/nitric oxide (NOS/NO) pathway in IN pathophysiology remains unclear.
Purpose of the Study:
- To investigate the involvement of the NOS/NO pathway in the development of intussusception.
- To explore the potential of modulating this pathway for IN prevention.
Main Methods:
- An intussusception (IN) rat model was induced using lipopolysaccharide (LPS).
- Nitrite/nitrate (NOx) levels, nitric oxide synthase (NOS) activity, and cyclic guanosine monophosphate (cGMP) concentrations were measured.
- The effects of L-arginine, sodium nitroprusside, and L-NAME on IN incidence were assessed.
Main Results:
- LPS administration induced IN in 40% of rats.
- L-arginine and sodium nitroprusside significantly increased IN incidence, while L-NAME (a NOS inhibitor) significantly decreased it.
- LPS elevated plasma NOx, tissue NOS activity, and cGMP levels, indicating increased NOS/NO pathway activation in IN.
Conclusions:
- The NOS/NO pathway plays a critical role in the pathophysiology of intussusception.
- Inhibition of NO production presents a potential therapeutic strategy for preventing intussusception.