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Effect of integrin on procollagen synthesis by fibroblasts from scleroderma
1Department of Dermatology, Changzheng Hospital, Second Military Medical University, Shanghai 200003, China.
Objective:
To investigate the effect of integrin on fibroblasts from scleroderma in the production of procollagen.
Methods:
Phosphorothioate modified antisense oligonucleotides were used to interfere with the expression of integrin alpha 5 or beta 1 subunit on fibroblasts from 10 cases of scleroderma, and then the changes of procollagen mRNA due to the decline in level of integrin were observed by using RT-PCR.
Results:
Expression of integrin alpha 5 or beta 1 subunit was specifically inhibited by their corresponding antisense oligonucleotides. Fibroblasts with decreased expression of alpha 5 or beta 1 subunit produced lower level of procollagen alpha 1 (I), alpha 1 (III) mRNA in comparison to the control.
Conclusion:
Overproduction of procollagen may be inhibited at the level of transcription by lowering the expression of integrin alpha 5 or beta 1 subunit on fibroblasts in scleroderma.