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Updated: Oct 3, 2026
Direct Reprogramming of Mouse Fibroblasts into Melanocytes
Published on: August 27, 2021
Modified technique of autologous noncultured epidermal cell transplantation for repigmenting vitiligo: a pilot study
N van Geel1, K Ongenae, M De Mil
1Department of Dermatology, Ghent University Hospital, Ghent, Belgium.
Background:
Several reports have demonstrated that grafting of autologous melanocytes from normally pigmented donor skin can be used for repigmentation of achromic macules in vitiligo.
Objective:
To investigate a modified approach in which noncultured autologous melanocytes and keratinocytes are grafted on superficially laser dermabraded vitiligo lesions in a suspension enriched with hyaluronic acid.
Methods:
Four patients with stable vitiligo were treated using a noncultured melanocyte-keratinocyte suspension. The cellular suspension was grafted on vitiliginous lesions previously dermabraded with a CO2 laser. To improve the viscosity and fixation of the cellular suspension hyaluronic acid was added. Three weeks after grafting, psoralen plus ultraviolet A (PUVA) or ultraviolet B (UVB) therapy was started. Residual leukodermic areas were subsequently retreated.
Results:
Repigmentation was observed within 2-4 weeks and continued to increase for 3 months after treatment. In all patients, 85-100% repigmentation was achieved. A temporary slight color mismatch was visible in all patients. The most homogeneous repigmentation was obtained 5 months after treatment.
Conclusion:
This modified procedure seems to be a simple and promising treatment for larger vitiliginous areas.
