Related Experiment Video
Updated: Aug 6, 2026
14:23
Generation of Induced Regulatory T Cells from Primary Human Naïve and Memory T Cells
Published on: April 16, 2012
CD4+CD25+ regulatory cells in acquired MHC tolerance
E H Field1, D Matesic, S Rigby
1Department of Veterans Affairs Medical Center, Iowa City, Iowa 52246, USA. liz-field@icva.gov
Immunological Reviews
|November 28, 2001
Summary
Regulatory CD4+CD25+ cells induce tolerance to foreign MHC antigens. These cells prevent the development of CD8+ T-cell responses, crucial for acquired self-tolerance.
Area of Science:
- Immunology
- Transplantation Immunology
Background:
- Self-tolerance is maintained by central and peripheral mechanisms.
- Regulatory CD4+ T cells are key in controlling immune responses to self-antigens.
- Neonatal exposure to foreign MHC antigens induces tolerance, serving as a model for acquired self-tolerance.
Purpose of the Study:
- To investigate the role of regulatory CD4+ T cells in acquired tolerance to foreign MHC antigens.
- To determine if regulatory CD4+ T cells can prevent T-cell alloreactivity.
Main Methods:
- Adoptive transfer of spleen cells from neonatally tolerant mice into SCID recipients.
- Assessment of skin graft rejection.
- In vitro assays including cytotoxic T-lymphocyte assays, ELISPOT, and flow cytometry to analyze CD8+ T-cell effector function.
Main Results:
- Adoptive transfer of unfractionated spleen cells from tolerant mice inhibited skin graft rejection.
- CD4+CD25+ cells from tolerant mice suppressed the development of anti-donor CD8+ T-cell responses in vitro.
- Evidence of CD4+CD25+ regulatory cells mediating tolerance to foreign MHC.
Conclusions:
- Regulatory CD4+CD25+ cells play a critical role in initiating and maintaining tolerance to foreign MHC antigens.
- These regulatory cells prevent the development of CD8+ T-cell alloreactivity, contributing to acquired self-tolerance.
Related Concept Videos
01:40Cell-mediated Immune Responses
Overview
01:20Special Features of Adaptive Immunity
The adaptive immune system, a crucial component of the overall immune response, offers a highly specialized defense against pathogens. It involves specific cell types and features, enabling it to combat infections effectively and efficiently.
The primary cell types involved in adaptive immunity are T cells and B cells. Each type has a unique role in defending the body against pathogens. T cells are responsible for cell-mediated immunity. They identify and eliminate infected cells directly,...
The primary cell types involved in adaptive immunity are T cells and B cells. Each type has a unique role in defending the body against pathogens. T cells are responsible for cell-mediated immunity. They identify and eliminate infected cells directly,...
01:22T Cell Activation and Clonal Selection
T cells are integral to our adaptive immune system, recognizing and effectively responding to foreign antigens. T cell activation and clonal selection are pivotal in orchestrating this immune response. This article elucidates these mechanisms, detailing the roles of cluster of differentiation (CD) markers, major histocompatibility complex (MHC) molecules, costimulatory signals, and the process of clonal selection.
Naive T cells that have not yet encountered an antigen express two primary CD...
Naive T cells that have not yet encountered an antigen express two primary CD...
01:24T Cell Types and Functions
When T cells with CD4 markers are activated, they give rise to two types of effector cells: helper T cells and regulatory T cells. Meanwhile, T cells with CD8 markers differentiate into effector cytotoxic T cells. The differentiation of CD4 T cells into helper T cell subsets, such as Th1, Th2, and Th17 cells, is dependent on the antigen type, antigen-presenting cell, and regulatory cytokines.
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
Th1 cells stimulate dendritic cells to express necessary co-stimulatory molecules on their surfaces for...
01:27Cytotoxic T Cells-mediated Immune Response
Cytotoxic T cells are a vital component of the immune system. They have the remarkable ability to identify and target antigens on infected or abnormal cells. These antigens often originate from intracellular pathogens such as viruses or abnormal proteins cancer cells produce.
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...
Immunological surveillance is the ability of immune cells to monitor and eliminate infected cells with intracellular pathogens, neoplastically transformed cells, and cells with non-self antigens. Cytotoxic T cells and NK...