Related Experiment Video
Updated: Aug 13, 2026
Biomimetic Materials to Characterize Bacteria-host Interactions
Published on: November 16, 2015
Adsorption of biosurfactant on solid surfaces and consequences regarding the bioadhesion of Listeria monocytogenes
T Meylheuc1, C J van Oss, M N Bellon-Fontaine
1Unité de Recherche en Bioadhésion et Hygiène des Matériaux, Institut National de la Recherche Agronomique, Massy, France. meylheuc@massy.inra.fr
Aims:
The influence of biosurfactant compounds produced by a strain of Pseudomonas fluorescens on the adhesion of Listeria monocytogenes LO28 to polytetrafluoroethylene (PTFE) and AISI 304 stainless steel surfaces was investigated.
Methods And Results:
The biosurfactant was produced according to a simple, novel technique based on cultivation on nutrient agar. Adhesion studies were performed using L. monocytogenes cells cultured at 20 or 37 degrees C.
Conclusions:
A substrate-dependent behaviour of the LO28 strain (larger number of cells adhering to stainless steel than to PTFE), and a significant reduction (< 90%) in microbial adhesion levels through the prior adsorption of biosurfactants on stainless steel surfaces, which can be related to a change in the electron-donor characteristics of this substratum, was demonstrated.
Significance And Impact Of The Study:
The prior adsorption of biosurfactants on solid surfaces may constitute a new and effective means of combating the implantation of pathogenic micro-organisms in food processing plants.
Related Concept Videos
01:18Surface Membrane Barriers
The outer layer of the skin, the epidermis, is a robust barrier comprising layers of closely packed keratinized cells. This dense arrangement prevents microbes from penetrating the body. The periodic shedding of epidermal cells...
01:29Biofilms