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Related Experiment Videos

Enterotoxigenic potential of Staphylococcus intermedius.

K Becker1, B Keller, C von Eiff

  • 1Institute of Medical Microbiology, University of Münster, 48149 Münster, Germany. kbecker@uni-muenster.de

Applied and Environmental Microbiology
|November 28, 2001
PubMed
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This study found that many Staphylococcus intermedius isolates, particularly from dogs, carry genes for staphylococcal enterotoxins. These bacteria can produce toxins, posing a risk for staphylococcal food poisoning (SFP) through food contamination.

Area of Science:

  • Microbiology
  • Food Safety
  • Genomics

Background:

  • Staphylococcal food poisoning (SFP) is a significant foodborne illness.
  • Enterotoxigenic Staphylococcus aureus is a known cause of SFP.
  • Limited genomic screening for enterotoxins in Staphylococcus intermedius.

Purpose of the Study:

  • To assess the enterotoxigenic potential of Staphylococcus intermedius isolates.
  • To screen for specific staphylococcal enterotoxin genes (sea, seb, sec, sed, see) in S. intermedius.
  • To compare molecular findings with in vitro enterotoxin production.

Main Methods:

  • Multiplex PCR DNA-enzyme immunoassay system used for gene detection.
  • Tested 281 veterinary and 11 human S. intermedius isolates.

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  • In vitro enterotoxin production confirmed by immunological tests.

    • Main Results:

    • 33 (11.3%) S. intermedius isolates tested positive for the sec gene.
    • 31 of these positive isolates were from canine sources.
    • 90.9% of sec-positive isolates produced enterotoxins in vitro.
    • No enterotoxigenic coagulase-negative staphylococci were detected.

    Conclusions:

    • A significant proportion of S. intermedius isolates possess enterotoxin production potential.
    • Evidence suggests S. intermedius can contribute to SFP via food contamination.
    • Potential for zoonotic transmission of enterotoxigenic S. intermedius from animals to humans.