Related Experiment Video
Updated: Aug 17, 2026
The Cell-based L-Glutathione Protection Assays to Study Endocytosis and Recycling of Plasma Membrane Proteins
Published on: December 13, 2013
Histochemical localization of glutathione dependent NBT-reductase in mouse skin
1Environmental Carcinogenesis Division, Industrial Toxicology Research Centre, Post Box 80, M. G. Marg, Lucknow-226 001, India. Yogeshwer_shukla@hotmail.com
Objective:
Localization of the glutathione dependent Nitroblue tetrazolium (NBT) reductase in fresh frozen sections of mouse skin and possible dependence of NBT reductase on tissue thiol levels has been investigated.
Methods:
The fresh frozen tissue sections (8 m thickness) were prepared and incubated in medium containing NBT, reduced glutathione (GSH) and phosphate buffer. The staining for GSH was performed with mercury orange.
Results:
The activity of the NBT-reductase in mouse skin has been found to be localized in the areas rich in glutathione and actively proliferating area of the skin.
Conclusion:
The activity of the NBT-reductase seems to be dependent on the glutathione contents.
More Related Videos
07:47Live Imaging of the Mitochondrial Glutathione Redox State in Primary Neurons using a Ratiometric Indicator
Published on: October 20, 2021
03:35Rapid Quantification of Oxidized and Reduced Forms of Glutathione Using Ortho -phthalaldehyde in Cultured Mammalian Cells In Vitro
Published on: June 28, 2024
