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Use of a polymerase chain reaction assay to detect infection with Eperythrozoon wenyoni in cattle
J M Vandervoort1, C Bourne, R L Carson
1Department of Small Animal Medicine and Surgery, College of Veterinary Medicine, University of Illinois, Urbana 61801, USA.
Objective:
To determine whether a polymerase chain reaction (PCR) assay could be used to detect Eperythrozoon wenyoni in the blood of cattle.
Design:
Prospective study.
Animals:
95 cattle from various herds in Alabama and Georgia and 96 bulls enrolled in Auburn University's Alabama Beef Cattle Improvement Association Bull Test program.
Procedure:
Blood samples were collected by means of venipuncture of the median caudal vein and submitted for a CBC and PCR assay. Blood smears were made immediately after blood collection and examined by means of light microscopy.
Results:
Three of 95 cattle from herds in Alabama and Georgia and 5 of 96 bulls enrolled in the Bull Test program had positive PCR assay results. Organisms were seen in blood smears from only 5 of these 8 animals. Organisms were not seen in blood smears from any animals for which results of the PCR assay were negative.
Conclusions And Clinical Relevance:
Results suggest that a PCR assay may be an effective method for detecting E wenyoni infection in cattle and that the PCR assay may be a more sensitive test than evaluation of blood smears.
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