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Identification of genes induced by taxol application using a combination of differential display RT-PCR and DNA
E Fukusaki1, T Oishi, H Tanaka
1Department of Biotechnology, Graduate School of Engineering, Osaka University, Suita, Japan. fukusaki@bio.eng.osaka-u.ac.jp
Zeitschrift Fur Naturforschung. C, Journal of Biosciences
|November 29, 2001
Abstract:
The differential display reverse transcriptional polymerase chain reaction (DD-RT-PCR) was used to hunt for cDNA fragments specifically expressed by taxol treatment of HeLa cells. Forty-eight cDNA clones were differentially displayed through the experiments. The cDNA fragments obtained were separately spotted onto glass slides to prepare a tailor-made DNA chip. The gene expression pattern of differentially displayed cDNA fragments were checked by DNA microarray analysis.