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Updated: Oct 3, 2026

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes
09:14

Static Adhesion Assay for the Study of Integrin Activation in T Lymphocytes

Published on: June 13, 2014

The lymphocyte metalloprotease MDC-L (ADAM 28) is a ligand for the integrin alpha4beta1

Lance C Bridges1, Patricia H Tani, Krista R Hanson

  • 1Department of Biochemistry and Molecular Biology, the University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma 73190, USA.

The Journal of Biological Chemistry
|November 29, 2001
PubMed

Insights

The disintegrin domain of lymphocyte ADAM 28 (MDC-L) binds to leukocyte integrin alpha(4)beta(1), mediating cell adhesion. This interaction, dependent on integrin activation, suggests MDC-L

Area of Science:

  • Immunology
  • Cell Biology
  • Biochemistry

Background:

  • Lymphocyte interactions are crucial for immune surveillance.
  • ADAM proteins, including MDC-L (ADAM 28), are involved in cell surface interactions.
  • Integrins, such as alpha(4)beta(1), mediate cell adhesion and migration.

Purpose of the Study:

  • To investigate the interaction between MDC-L and leukocyte integrins.
  • To characterize the role of the MDC-L disintegrin domain in cell adhesion.
  • To elucidate the functional implications of MDC-L-integrin binding.

Main Methods:

  • Utilized recombinant Fc fusion proteins of the MDC-L disintegrin domain.
  • Employed T-lymphoma cell lines (Jurkat) and transfected K562 cells.
  • Applied monoclonal antibodies (mAbs) against MDC-L and integrin subunits.
  • Investigated concentration-dependent and saturable binding kinetics.

Main Results:

  • The MDC-L disintegrin domain directly binds to the leukocyte integrin alpha(4)beta(1).
  • This binding supports Jurkat cell adhesion in a cation-dependent manner.
  • Adhesion was inhibited by anti-MDC-L and anti-integrin mAbs, and by the CS-1 peptide.
  • Binding required activation of lymphocyte beta(1) integrins.

Conclusions:

  • MDC-L interacts with alpha(4)beta(1) on leukocytes via its disintegrin domain.
  • This interaction may regulate metalloprotease activity and lymphocyte interactions.
  • MDC-L plays a potential role in leukocyte-leukocyte interactions mediated by alpha(4)beta(1).

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